Theory of the dynamic aspects of ultracentrifugation

Computer generated concentration distributions have allowed the development of numerical techniques for the determination of sedimentation and diffusion coefficients from the integrated Lamm equation; they are applicable to two component systems with minimal dependence of the characteristic parameters on concentration. Accuracy is assessed by comparison of the numerically estimated values of the relevant functions with their analytical values, as predicted by the analytical solutions of the Mason-Weaver equation. These methods theoretically allow simultaneous calculation of s and D to within 0.5% for a wide range of computer simulated data. The effects of solute heterogenity on the results are also detailed. (August 1973)