Experimental determination of sedimentation and diffusion coefficients employing concentration changes as a function of time

Numerical methods have been developed for rapid simultaneous determination of sedimentation and diffusion coefficients from transient state sedimentation analyses based on computer simulated data.  Application of this methodology to experimental data obtained for ribonuclease A and chymotrypsinogen A demonstrates that the values of the sedimentation and diffusion coefficients determined from this approach do not differ significantly from the values of these parameters obtained independently by conventional techniques.  Such determinations allow rapid estimation of apparent molecular weights. (August 1973)